Sharmila Shankar1, Suthakar Ganapathy1, Sunil R. Hingorani2, Rakesh K. Srivastava1
1Department of Biochemistry, University of Texas Health Science Center at Tyler, Tyler, Texas, USA 75703, 2Clinical Research Division, Fred Hutchinson Cancer Research Center, University of Washington School of Medicine, Seattle, WA 98109
TABLE OF CONTENTS1. Abstract2. Introduction3. Materials and Methods3.1. Reagents3.2. Cell culture and cell survival assay3.3. Capillary tube formation assay3.4. In vitro cell migration (invasion) assay3.5. In vivo studies3.6. Immunohistochemistry3.7. TUNEL assay3.8. Measurement of circulating endothelial cells3.9. Statistical analysis4. Results4.1. EGCG inhibits viability of HUVEC4.2. EGCG inhibits capillary tube formation by blocking ERK activity4.3. EGCG inhibits migration of HUVEC and AsPC-1 cells4.4. EGCG inhibits matrix metalloproteinases in vitro4.5. EGCG inhibits growth of pancreatic tumor cells xenografted in nude mice4.6. EGCG induces apoptosis in tumor tissues4.7. EGCG inhibits proliferation and causes growth arrest in tumor tissues4.8. EGCG inhibits ERK1/2 activity and induced JNK1/2 activity4.9. EGCG inhibits angiogenesis4.10. EGCG inhibits metastasis5. Discussion6. Acknowledgements7. References
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